Western Blot was performed using Anti-LTBR Polyclonal Antibody (Product # PA5-88290) and a 55 kDa band corresponding to Tumor necrosis factor receptor superfamily member 3 was observed across the cell lines tested except in SH-SY5Y and HEK-293 which are reported to be negative. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), MCF7 (Lane 3), HUVEC (Lane 4), SH-SY5Y (Lane 5) and HEK-293 (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Western Blot was performed using Anti-LTBR Polyclonal Antibody (Product # PA5-88290) and a 55 kDa band corresponding to Tumor necrosis factor receptor superfamily member 3 was observed across the cell lines tested except in SH-SY5Y and HEK-293 which are reported to be negative. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), MCF7 (Lane 3), HUVEC (Lane 4), SH-SY5Y (Lane 5) and HEK-293 (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supplier Page from Thermo Fisher Scientific for LTBR Antibody